In order to confirm the identity of blood, there are certain crystal and spectroscopic tests that are regularly employed in the forensic science laboratories.
1. Takayama Test:
Standard Glucose Solution (100g/100ml) 3 ml
10% Sodium hydroxide (NaOH) 3 ml
Pyridine 3 ml
Distilled Water 7 ml
Reagents should be made fresh daily.
1. Place material to be tested on a microscopic slide and cover with a cover slip.
2. Add a drop of Takayama Reagentand allow to flow under the cover slip.
3. Warm slide gently at 65oC for 10-20 seconds
4. Allow to cool and observe under microscope at 100X magnification.
The appearance of pink needle shaped crystals of pyridine hemochromogen (Pyridine ferroprotoprophyrin) is positive reaction for heme.
2. Teichmann’s Test:
Potassium Chloride or 0.1 g